physiological and biochemical data in model rats of congenital stationary night blindness

    AN Jing，GUO Qun，GU YongHao，LI Li，YAN GuoLin，ZHU DeSheng，SHI XinYou，ZHANG ZuoMing

    Department of Clinical Medicine, School of Aerospace Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To determine the basic physiological and biochemical data in a natural occurring mutant rats of congenital stationary night blindness(CSNB). METHODS: Forty CSNB rats in F11 and F12 (20 female and 20 male) and 5 SD rats, aged from 13 to 15 weeks, were examined by electroretinogram (ERG) technique to evaluate the inherited traits under anaesthesia. The blood was then collected from the abdominal vein in torrefied tubes to measure the biochemical parameters. Then heart, liver, spleen, lung, brain (cerebrum, cerebellum, brain stem), pituitary, kidney, adrenal gland and testicle were taken to weigh. RESULTS: There were significant differences in organ weight between two sexes in CSNB rat (P<0.05), except for spleen,adrenal gland, pituitary. The coefficients of organs were significantly higher in female CSNB rats than that of the male except for kidney (P<0.01). The GLU, CHO, ALB were higher, and ALT, AST, ALP, BUN, CRE, TG, TP were lower in female rats; the result of electrolyte was that K, Na and iCa were higher in the male, Cl was higher in female, but there were no significant differences between female and male in statistics (P>0.05). CONCLUSION: The basic physiological and biochemical data of CSNB rat strain are in normal range. There are significant differences in some basic physiological and biochemical data between female and male CSNB rats, but not lots between CSNB rats and control SD rats. CSNB rat will become a new mutant model rat for researches on inherited retinal disease.

    【Keywords】 night blindness/congenital; rat; electroretinogram; physiology; biochemistry

    【摘要】 目的：对本实验室首先发现并培育的自发性先天性静止性夜盲(CSNB)大鼠的主要脏器及血液、生化指标进行检测. 方法：随机选取出生13～15 wk的第11，12代CSNB大鼠通过视网膜电图鉴定后，抽取静脉血进行血清生化相关指标的检测，并对其重要脏器进行准确称量. 结果： CSNB大鼠脏器质量在雌雄之间除脾脏、肾上腺、脑垂体外，其余均存在显著差异(P<0.05)；脏器系数大脑、小脑、脑干雌雄间存在非常显著差异(P<0.01)；生化指标葡萄糖、总胆固醇和白蛋白在雌性大于雄性，丙氨酸基转换酶、天门冬氨酸基转换酶、碱性磷酸酶、尿素氮、肌酐、甘油三酯和总蛋白在雌性小于雄性；血电解质K+，Na+，离子钙(iCa)在雄性大于雌性， 雌性的Cl-值高，但无差异. 结论： CSNB大鼠基本生理学和生物化学参数均在正常范围内，其中一些参数在CSNB大鼠性别之间存在差异,但是与正常对照SD大鼠之间只有个别参数有差异. CSNB大鼠为遗传性视网膜疾病的研究提供了一种新的突变模式动物品系.

    【关键词】 夜盲/先天性；大鼠；视网膜电图；生理学；生物化学

    0引言

    遗传性视网膜疾病是致盲的重要原因，目前缺乏有效的治疗手段. 模式动物是研究基因和蛋白功能的重要支撑条件，也是研究疾病防治和药物开发的重要手段. 目前，日本京都大学正在进行一项生物资源保存计划，与眼科相关的有6种. 美国Jackson实验室是目前世界上最大的突变小鼠库，该实验室先后发现16种自发性基因突变视网膜疾病模式小鼠[1]. 我们在实验中发现一种视网膜电图(ERG)异常大鼠，经遗传学和视觉电生理学鉴定，确定为一种类似人类先天性静止性夜盲(congenital stationary night blindness, CSNB)模式大鼠[2-3]，并已经近交繁育至14代. 为建立一种新的、具有我国自主知识产权的视网膜疾病模式动物品系，我们对该品系的大鼠基本生理学及生物化学参数进行了测量.